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polyacrylamide bis tris minigels  (Thermo Fisher)


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    Thermo Fisher polyacrylamide bis tris minigels
    Polyacrylamide Bis Tris Minigels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyacrylamide+bis+tris+minigels/TRIS-HCL/10__1038_slash_s42005___024___01741___3-260-14-19
    Average 99 stars, based on 1 article reviews
    polyacrylamide bis tris minigels - by Bioz Stars, 2026-10
    99/100 stars

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    Electrophoresis:

    Article Title: Cell stiffening is a label-free indicator of reactive oxygen species-induced intracellular acidification
    Article Snippet: Samples containing 2.5 μg of protein were prepared by adding 13 μL of protein extract diluted in PBS to 5 μL sample buffer (Novex, NuPAGE LDS-Sample Buffer4x, ThermoFisher Scientific) and 2 μL of sample reducing agent (NuPAGE Sample Reducing Agent10x, ThermoFisher Scientific). .. Samples were subsequently heated to 70 °C for 10 min before electrophoresis on 4–12% polyacrylamide Bis-Tris minigels (NuPAGE system, ThermoFisher Scientific). .. After electrophoresis in a XCell SureLock MiniCell (Invitrogen ThermoFisher Scientific), the gel was electro-blotted to nitrocellulose membranes (Amersham Protran 0,2 μm, GE Healthcare) using the Trans-Blot Semi-Dry cell (Bio-Rad), whichwere then stainedwith 0.5% (w/v) Ponceau S (AcrosOrganics) and imaged using the Chemi-Smart-5100 system (Vilber, Peqlab Biotechnologie GmbH) together with the Chemi-Smart software (version 15.01a) to assess protein load on each lane.



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